Sage-Tips

Why is locating an object more difficult if you start with the high power objectives than with the low power objective?

Why is locating an object more difficult if you start with the high-power objective than with the low-power objective? Because the high power brings the object closer so it might be difficult to focus.

Why should the coarse adjustment knob not be used when the HPO and OIO objectives are set in place?

Coarse and fine adjustment The coarse adjustment knob should only be used with the lowest power objective lens. Once it is in focus, you will only need to use the fine focus. Using the coarse focus with higher lenses may result in crashing the lens into the slide.

Why do you need to focus specimen under the LPO first before the HPO?

Using the low power objective (LPO) gives us an overview of a specimen. If there is anything specific you want to observe, it would be easier to identify it first using the LPO and then zooming in on it using the high power objective (HPO).

Why should you use the low power objective first in locating specimen instead of high power?

The field of view is largest on the lowest power objective. When you switch to a higher power, the field of view closes in towards the center. You will see more of an object on low power. Therefore, it is best to find an object on low power, center it, and then switch to the next higher power and repeat.

Why is it easier to view the cells using the low power objective lens first?

When using a light microscope it’s important to start with the low power objective lens as the field of view will be wider, increasing the number of cells you are able to see. This makes it easier to find what you’re looking for.

When looking at a specimen on your slide you should always start with the?

When focusing on a slide, ALWAYS start with either the 4X or 10X objective. Once you have the object in focus, then switch to the next higher power objective. Re-focus on the image and then switch to the next highest power.

Why should not use the coarse adjustment knob if you observe under HPO?

Use ONLY the fine adjustment knob to focus on high power. NEVER USE THE COARSE FOCUS KNOB ON HIGH POWER! The high power lens should be very close to your slide when in proper focus. If you turn the coarse adjustment knob while on high power, the objective could easily break your slide.

Why must you not use the coarse focus knob of a microscope when looking through the 10x 40x or 100x objective lenses?

If you focus the high power objectives with the coarse focus knob, then you risk slamming the objective into the slide, risking the damage of both. Some high power objectives are spring-loaded, with a front-part that retracts when touched by the slide. This is a protective mechanism.

Why would you want to wet mount a specimen?

Why would use a wet mount? To increase the specimens translucency and to make it easier to stain. Using a wet mount slide has the tendency to flatten the specimen making it easier to view.

Which objective should I start with when focusing on a specimen?

3. When focusing on a slide, ALWAYS start with either the 4X or 10X objective. Once you have the object in focus, then switch to the next higher power objective. Re-focus on the image and then switch to the next highest power.

What objective should you use to initially locate the specimen Why?

Start Low! Since the 4x objective lens has the least magnification, but a larger field of view, it allows for more of the specimen to be seen, as well as locating the part of the sample you wish to view. This in turn makes it easier to focus on the sample.

When should you use the low power objective lens on the microscope?

Low power objectives cover a wide field of view and they are useful for examining large specimens or surveying many smaller specimens. This objective is useful for aligning the microscope. The power for the low objective is 10X.

Why should you start with the lowest magnification objective when getting a specimen?

Why Should You Start With the Lowest Magnification Objective When Getting a Specimen in Focus? Microscopes can be tricky to use because of the strength of their lens magnifications, typically 4x, 10x and 40x.

Why are microscopes so difficult to use?

Microscopes can be tricky to use because of the strength of their lens magnifications, typically 4x, 10x and 40x. The difficulty lies in trying to align the specimen in a precise position so the microscope lens can capture it and allow the user to see the specimen close up.

Why is it difficult to use high magnification lenses?

When magnification is increased, the specimen must be recentered and refocused carefully. Since the field of vision gets smaller and smaller with each increase, the difficulty of using these lenses is having to “chase” or reposition the specimen in the right line of sight.