Table of Contents
- 1 Which of the following are examples of strong exchangers for ion exchange chromatography?
- 2 What are strong and weak ion exchange resins?
- 3 Which is example of stronger anion exchanger?
- 4 What are the applications of ion exchange chromatography in clinical laboratory diagnostics?
- 5 What is the function of weak base anion exchanger?
- 6 How does ion exchange chromatography improve resolution?
Which of the following are examples of strong exchangers for ion exchange chromatography?
Some examples of functional groups of strong ion exchange resins are quaternary ammonium cation (Q), which is an anion exchanger, and sulfonic acid (S, -SO2OH), which is a cation exchanger. These types of exchangers can maintain their charge density over a pH range of 0–14.
What are the applications of ion exchange chromatography?
Ion exchange chromatography can be applied for the separation and purification of many charged or ionizable molecules such as proteins, peptides, enzymes, nucleotides, DNA, antibiotics, vitamins and etc. from natural sources or synthetic origin.
What are strong and weak ion exchange resins?
The terms “strong” and “weak” refer to the acid/base properties of the functional group. If the ligand is derived from a strong acid or a strong base it is referred to as a strong ion exchange resin. If the ligand is derived from a weak acid or a weak base it is referred to as a weak ion exchange resin.
What is the role of ion exchanger?
Ion exchangers are either cation exchangers that exchange positively charged ions (cations) or anion exchangers that exchange negatively charged ions (anions). There are also amphoteric exchangers that can exchange both cations and anions simultaneously.
Which is example of stronger anion exchanger?
Examples of strong ion exchangers are quaternary ammonium (Q), sulfonate (S), and sulfopropyl (SP) resins. Strong- cation exchange resins typically feature sulfonic acid functional groups while Strong anionic- exchange resins contain quaternary amines.
What are the advantages and disadvantages of ion exchange chromatography?
Pros and Cons of Ion Exchange Chromatography
| IEX Pros | IEX Cons |
|---|---|
| Concentrates samples | Clusters of positively charged residues can cause a net-negatively charged protein to bind a cation exchanger, and vice versa |
| High yield | Small changes in pH can greatly alter binding profile of IEX resin |
What are the applications of ion exchange chromatography in clinical laboratory diagnostics?
It provides: availability of high capacity stationary phases and sensitive detectors; simple sample preparation; avoidance of hazardous chemicals; decreased sample volumes; flexible reaction options on a changing sample matrix to be analyzed; or the option to operate a fully-automated system.
What is strong ion exchanger?
Based on differences in the functional groups, ion exchangers may be classified into strong and weak exchangers. Strong ion exchangers are fully ionized over the entire operating pH, whereas the degree of ionization of weak exchangers is pH dependent.
What is the function of weak base anion exchanger?
Weak base anions are more chemically stable than strong base anions and are used for the removal of mineral acids, organic acids and other organic materials. They are highly resistant to organic fouling.
What are ion exchangers in ion exchange chromatography?
Ion exchangers are solid materials or liquid solutions which are able to take up (or absorb) positively or negatively charged ions from aqueous electrolyte solutions and at the same time release other ions of equivalent amount into the aqueous solution.
How does ion exchange chromatography improve resolution?
The easiest way to improve the resolution from an IEX run is to modify the running conditions. Different pH will affect the charged surface on the protein, which affects the resolution. A smaller amount of sample will improve the resolution: typically use up to 30\% of the complete capacity to maintain good resolution.
What is strong anion exchange chromatography?
Anion exchange chromatography is a form of ion exchange chromatography (IEX), which is used to separate molecules based on their net surface charge. Anion exchange chromatography, more specifically, uses a positively charged ion exchange resin with an affinity for molecules having net negative surface charges.