Table of Contents
- 1 How many DNA fragments are formed from digest a if ecor1 cuts the Digest?
- 2 How many fragments pieces would you expect if you cut the linear lambda DNA with HindIII alone?
- 3 How many fragments are produced by PstI?
- 4 What are fragments of DNA?
- 5 What is the composition of a DNA fragment that is what is a DNA fragment made of?
- 6 How many fragments are produced by EcoRI?
- 7 Can restriction enzymes predict the size of DNA fragments produced?
How many DNA fragments are formed from digest a if ecor1 cuts the Digest?
When cut by EcoRI, the cDNA will yield 0.5, 1.5, and 2 kilobase fragments. Digesting with both EcoRI and HindIII, will yield 0.5, 1, and 1.5 kilobase fragments.
How many fragments pieces would you expect if you cut the linear lambda DNA with HindIII alone?
The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1).
How many fragments are produced by PstI?
c) For those two restriction endonucleases, each DNA fragment in the single digest is cut by PstI into two DNA fragments in the double digest (i.e. the restriction endonuclease plus PstI).
What happens when the vector DNA is digested with EcoRI?
A plasmid vector is digested with EcoRI at a single site to produce two sticky ends. A sample of human DNA is also digested with EcoRI to produce pieces with the same sticky ends. DNA ligase is used to covalently link the fragments.
How are the DNA fragments separated?
Gel electrophoresis is a technique used to separate DNA fragments (or other macromolecules, such as RNA and proteins) based on their size and charge. All DNA molecules have the same amount of charge per mass. Because of this, gel electrophoresis of DNA fragments separates them based on size only.
What are fragments of DNA?
DNA fragmentation is the separation or breaking of DNA strands into pieces. It can be done intentionally by laboratory personnel or by cells, or can occur spontaneously. Spontaneous or accidental DNA fragmentation is fragmentation that gradually accumulates in a cell.
What is the composition of a DNA fragment that is what is a DNA fragment made of?
nucleotides
What is DNA made of? DNA is made up of molecules called nucleotides. Each nucleotide contains a phosphate group, a sugar group and a nitrogen base. The four types of nitrogen bases are adenine (A), thymine (T), guanine (G) and cytosine (C).
How many fragments are produced by EcoRI?
How many fragments are produced by EcoRI? After digestion with EcoRI, you obtain four fragments: 1, 2, 3, and 4. After digestion of each of these fragments with HindII, you find that fragment 3 yields two subfragments (31 and 32) and that fragment 2 yields three (21, 22, and 23).
What is the size of the complete digestion of DNA?
Complete digestion with Y = 1 fragment of 1000 bp (single site cutting of a circular DNA, linearizes it). In Above image, there is one Sal I site and two Kpn I site. Complete digestion with Y = 1 fragment of 1000 bp (single site cutting of a circular DNA, linearizes it).
How many EcoR1 sites are there in the human genome?
If DNA is linear then the number of fragments generated is (N+1), where N= number of recognition sites or sequences. Hence the number of fragments generated is 5 if we digest a linear DNA molecule with a restriction enzyme having four recognition sites on the DNA. Also, how many ecor1 sites are in the human genome? 732421
Can restriction enzymes predict the size of DNA fragments produced?
Since the whole sequence of λ is already known we can predict where each restriction enzyme will cut and thus the expected size of the fragments that will be produced. If the virus DNA is exposed to the restriction enzyme for only a short time, then not every restriction site will be cut by the enzyme.