Table of Contents
- 1 Are cell culture experiments paired?
- 2 How many independent experiments are there?
- 3 What does N mean in experimental design?
- 4 Does cell culture count as in vivo?
- 5 What is an independent experiment?
- 6 What is K and N in statistics?
- 7 What is it called when an experiment can be repeated?
- 8 How to choose the right cell line for your research?
- 9 What is important when publishing a new cell line?
Are cell culture experiments paired?
Hi Julie, the same cells (eg primary cells from the same patient) divided into two groups, untreated and treated form a pair. You can use paired t-test to calculate if there’s any difference between them after treatment.
How many independent experiments are there?
How many independent experiments should you do? As many as possible really, but three is an achievable minimum for cell biology work. The number will generally be determined along empirical lines, with certain assays/techniques being more amenable to higher sample sizes than others.
What does N mean in experimental design?
In statistical studies at least, the value N (capitalized) equals population size and the value n (lowercase) is the sample size. The sample size is basically a certain amount of individuals in a given population used in an experiment in order to establish or recognize a greater trend.
What is the n value in an experiment?
N is the total number of individuals or units in the study (minus 1)
Why do you repeat experiments 3 times?
Repeating an experiment more than once helps determine if the data was a fluke, or represents the normal case. It helps guard against jumping to conclusions without enough evidence.
Does cell culture count as in vivo?
Actually, “in vivo” means “in living organism”, not “in living cell”. In vivo experiment is within living organism so the cell culture should be considered as in vitro and not in vivo.
What is an independent experiment?
Independent experiments: same or different experiments may be run in a sequence, with the sequence of outcomes being the object of interest. The trials – the individual experiments – may or may not affect the outcomes of later trials. If they do, the experiments are called dependent; otherwise, they are independent.
What is K and N in statistics?
N is the total number of cases in all groups and k is the number of different groups to which the sampled cases belong.
How many samples do I need for 95 confidence?
Remember that z for a 95\% confidence level is 1.96. Refer to the table provided in the confidence level section for z scores of a range of confidence levels. Thus, for the case above, a sample size of at least 385 people would be necessary.
Why must experiments be repeatable?
Why is the ability to repeat experiments important? Replication lets you see patterns and trends in your results. This is affirmative for your work, making it stronger and better able to support your claims. This helps maintain integrity of data.
What is it called when an experiment can be repeated?
Getting the same result when an experiment is repeated is called replication. Replication is important in science so scientists can “check their work.” The result of an investigation is not likely to be well accepted unless the investigation is repeated many times and the same result is always obtained.
How to choose the right cell line for your research?
In short, base your choice of human primary cells or immortal cell lines on your research aim. In many cases, immortal cell lines offer a very valuable model for preliminary experiments. Then use human primary cells to replicate key findings.
What is important when publishing a new cell line?
Indeed, a detailed characterization, the immortality of the culture, a proof of neoplasticity, authentication of the true origin of the cells, scientific significance and availability of the cell line for other investigators are of paramount importance when publishing a new cell line.
Can cell lines be used as a model system?
This article has been cited byother articles in PMC. Abstract Cell lines are in vitro model systems that are widely used in different fields of medical research, especially basic cancer research and drug discovery. Their usefulness is primarily linked to their ability to provide an indefinite source of biological material for experimental purposes.
Why is it so difficult to establish a new cell line?
However, many causes of this difficulty and serendipity for the establishment of a new cell line can be understood by taking into consideration the extreme differences (such as growth factor dependence, the percentage of oxygen, interaction with the stroma and immune cells, etc.) that exist between the in vivo and in vitro microenvironments [3].