Table of Contents
Why is lambda DNA used as a marker?
Lambda DNA (48,502 bp) may be used as a molecular weight size marker during nucleic acid gel analysis following digestion with a restriction enzyme (such as HindIII). Lambda DNA can also be used as a substrate in restriction enzyme activity assays.
Why is lambda phage important?
Lambda phage has been of major importance in the study of specialized transduction. Specialized transduction is the process by which a restricted set of bacterial genes are transferred to another bacterium. The genes that get transferred (donor genes) depend on where the phage genome is located on the chromosome.
How is lambda phage used?
Lambda phage is a non-contractile tailed phage, meaning during an infection event it cannot ‘force’ its DNA through a bacterial cell membrane. It must instead use an existing pathway to invade the host cell, having evolved the tip of its tail to interact with a specific pore to allow entry of its DNA to the hosts.
Why lambda DNA is used in restriction digestion?
Since the lambda genome is approximately 48,000 base pairs, each restriction enzyme will cut the DNA several times and generate restriction fragments of different sizes. In this activity, three separate samples of lambda DNA will be cut using three different restriction enzymes, and one sample will remain undigested.
What is lambda marker?
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer.
What is phage lambda DNA?
Phage lambda DNA is a common substrate for restriction endonucleases and for generating DNA size marker fragments. For large scale isolation of phage DNA, cI857Sam7, a mutant carrying four known mutations, is used. The DNA sequence used to construct a phage alpha restriction map includes these mutations. Applications.
What are all of the sizes of the pieces you expect if you cut lambda DNA with HindIII alone?
Cutting with Hin dIII will yield fragments of 1, 2, and 2.5 kilobases. Cutting with Eco RI will yield fragments of 1.5, 2, and 3.5 kilobases. When cut by EcoRI, the cDNA will yield 0.5, 1.5, and 2 kilobase fragments. Digesting with both EcoRI and HindIII, will yield 0.5, 1, and 1.5 kilobase fragments.
What is the HindIII lambda phage size marker?
What is the size of lambda phage?
Thermo Scientific Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 bp) with 12 bp single-stranded complementary 5′-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining.
How is lambda used in biotechnology?
Bacteriophages such as lambda phages can also be used as effective vectors for transferring recombinant DNA molecules into cells for cloning. A lambda phage is a bacteriophage that infects E. Coli cells. We can extract the phage’s DNA and remove a piece of their DNA and replace it with the DNA fragment to be cloned.
How is the lambda DNA marker digested and purified?
Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme (s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker. The marker can be labeled radioactively with T4 Polynucleotide Kinase.
What is lambda DNA/HindIII marker?
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme (s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on…
Is lambda DNA a phage?
Lambda DNA Lambda DNA, a linear, double-stranded phage DNA containing 12 bp single-stranded complementary 5′-ends, is derived from an Escherichia coli bacteriophage (Bacteriophage lambda cI 857 Sam7).
What is the Thermo Scientific lambda DNA marker?
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme (s) and purified and dissolved in storage buffer.