Table of Contents
- 1 Why is c-fos used as a marker?
- 2 What is c-fos expression?
- 3 Is c-Fos a transcription factor?
- 4 What activates c-Fos?
- 5 What is FOS activation?
- 6 How is transcription factor c-jun regulated?
- 7 Is c-fos a marker for early gene expression in neurons?
- 8 Can c‐Fos be used as a transynaptic marker for nociresponsive activity?
Why is c-fos used as a marker?
C-fos is a proto-oncogene that is expressed within some neurons following depolarization. The protein product, c-fos protein, can be identified by immunohistochemical techniques. Therefore, c-fos expression might be used as a marker for neuronal activity throughout the neuraxis following peripheral stimulation.
What does c-fos do in the brain?
c-Fos is currently used as a marker of neuronal activity and has been associated with a number of neural and behavioral responses to acute stimuli expression.
What is c-fos expression?
Expression of c-fos is an indirect marker of neuronal activity because c-fos is often expressed when neurons fire action potentials. Upregulation of c-fos mRNA in a neuron indicates recent activity. The c-fos promoter has also been utilised for drug abuse research.
What is FOS neuroscience?
The detection of the Fos protein, a product of the cfos gene, within neurons is a common procedure used in behavioral neuroscience research to identify neurons and circuits within the brain that are active during certain behavioral situations.
Is c-Fos a transcription factor?
Although c-Fos and other IEGs ultimately function as transcription factors and regulate downstream target genes, c-Fos has most widely been used as a functional marker of activity in neurons and neuronal circuitries after a variety of stimuli.
What does C Jun stand for?
c-Jun is a protein that in humans is encoded by the JUN gene. c-Jun, in combination with c-Fos, forms the AP-1 early response transcription factor. It was first identified as the Fos-binding protein p39 and only later rediscovered as the product of the JUN gene.
What activates c-Fos?
Intracellular Signaling The c-fos gene is activated rapidly by neurotransmitters or drugs that stimulate the cAMP pathway or Ca2+ elevation. Both pathways produce phosphorylation of transcription factor CREB. The c-fos gene contains three binding sites for CREB.
Are c-Fos nuclear?
from what I have seen in the basolateral amygdala, c-Fos staining is generally nuclear, but I have seen also more cytoplasmic signal, particularly in some interneuron populations. We used an anti-c-Fos antibody raised in rabbit (1:500; catalog #ab7963, Abcam) revealed with an anti-rabbit Cy3 secondary (1:500).
What is FOS activation?
Activation of the c-fos Gene The c-fos gene is activated rapidly by neurotransmitters or drugs that stimulate the cAMP pathway or Ca2+ elevation. Both pathways produce phosphorylation of transcription factor CREB. The c-fos gene contains three binding sites for CREB.
What are Jun and Fos?
The fos and jun proto-oncogenes are members of the set of genes known as cellular immediate-early genes. Their expression is induced transiently by a great variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons.
How is transcription factor c-jun regulated?
The transcriptional activities of c-Jun, ATFs, and MEF2 are regulated upon phosphorylation by various protein kinases, including the mitogen-activated protein kinases (MAPK), which have been implicated in vitro in the transcriptional regulation of c-jun (18, 23).
What gene do Fos and Jun regulate?
Is c-fos a marker for early gene expression in neurons?
In our studies, we focus primarily on expression of one immediate early gene product, the c-Fos protein. We also include some experiments using expression of other members of the Fos family and Jun proteins as markers for neuronal activation.
What is the function of c-Fos?
C-fos is a proto-oncogene that is expressed within some neurons following depolarization. The protein product, c-fos protein, can be identified by immunohistochemical techniques. Therefore, c-fos expression might be used as a marker for neuronal activity throughout the neuraxis following peripheral …
Can c‐Fos be used as a transynaptic marker for nociresponsive activity?
The data suggest that c‐fos can be used as a transynaptic marker for neuronal activity following noxious stimulation. However, c‐fos is expressed only in some kinds of neurons following peripheral stimulation, and it therefore may be an incomplete marker for nociresponsive activity.
Is c-fos a proto-oncogene?
Expression of c-fos-like protein as a marker for neuronal activity following noxious stimulation in the rat C-fos is a proto-oncogene that is expressed within some neurons following depolarization. The protein product, c-fos protein, can be identified by immunohistochemical techniques.