Table of Contents
What does mCherry tag?
mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). Fluorescent proteins are used to tag components in the cell, so they can be studied using fluorescence spectroscopy and fluorescence microscopy. mCherry absorbs light between 540-590 nm and emits light in the range of 550-650 nm.
Can GFP bind to DNA?
An oligomerization-dependent system comprising two fragments of green fluorescent protein (GFP) fused to engineered zinc fingers could be a means to detect virtually any double-stranded DNA sequence.
What is mCherry plasmid?
mCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed. mCherry matures rapidly, making it possible to see results very soon after transfection or activation of transcription. It is highly photostable and resistant to photobleaching (Shaner et al. 2004).
What is mCherry mRNA?
Description. mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.
What is mCherry fluorescence?
mCherry is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a very rapidly-maturing monomer with low acid sensitivity.
Is mCherry a reporter gene?
Our results identified mCherry as a reliable and quantitative reporter for the study of gene expression in chloroplasts, which is essential for the biotechnological application of microalgal chloroplasts and for improved production of valuable recombinant proteins.
What does GFP bind?
Biologists use GFP as a marker protein. GFP can attach to and mark another protein with fluorescence, enabling scientists to see the presence of the particular protein in an organic structure. Gfp refers to the gene that produces green fluorescent protein.
What controls expression of the GFP gene?
The protein is coded for by a single gene. The GFP gene can be inserted downstream of the promoter of a gene in another organism. RNA polymerase binds to promoter regions to initiate transcription. If the GFP gene is inserted correctly, it can be expressed in organisms other than jellyfish.
Is mCherry a dimer?
We found that the brightness values of homo-dimers (normalized to the brightness of the corresponding monomer) for three widely used FPs, namely mEGFP (εdimer = 1.69 ± 0.05), mEYFP (εdimer = 1.63 ± 0.05) and mCherry (εdimer = 1.41 ± 0.04), are generally lower than two, indicating the presence of non-fluorescent …
Where is mCherry from?
sea anemones
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in transfection and transgenic experiments.
How does GFP bind to protein?
In the jellyfish, GFP interacts with another protein, called aequorin, which emits blue light when added with calcium. GFP can attach to and mark another protein with fluorescence, enabling scientists to see the presence of the particular protein in an organic structure.
What is mCherry and mCherry?
Created with sketchtool. Created with sketchtool. MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm.
How to tag other proteins in mCherry?
To tag other proteins, you gotta fuse the mCherry sequence with the protein you want to study, then when the fusion its expressed your tagged protein can be seen (all of this inside an expression vector).
What is the structure of recrecombinant mCherry?
Recombinant mCherry is expressed and purified from transformed E. coli. The protein is a 28.8 kDa monomer of 236 amino acids with a peak fluorescent excitation and emission at 587 nm and 610 nm, respectively.
What is the difference between mCherry and DsRed?
mCherry and the majority of red fluorescent proteins derive from the protein drFP583 (commonly known as DsRed) isolated from Discosoma sea anemones, while other fluorescent proteins in the green range are often variants of jellyfish GFP. DsRed is a tetramer optimally excited at 558 nm and has an emission maximum at 583 nm.