Table of Contents
What are the non structural proteins of dengue virus?
The 11kb RNA genome of the dengue virus encodes three structural proteins (envelope, pre-membrane, capsid) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5), all of which are translated as a single polyprotein that is subsequently cleaved by viral and host cellular proteases at specific …
What is NS1 protein in dengue?
Dengue virus (DENV) nonstructural protein 1 (NS1) is a highly conserved 46-kDa protein that contains 2 glycosylation sites (Asn-130 and Asn-207) and 12 conserved cysteine (Cys) residues. Here, we performed site-directed mutagenesis to generate systematic mutants of viral strain TSV01.
Why is the NS1 protein important?
The NS1 protein acts as a scaffolding protein that anchors the replication complex to the endoplasmic reticulum (ER) membrane and interacts physically with NS4B (4).
How many dengue serotypes are there?
The virus responsible for causing dengue, is called dengue virus (DENV). There are four DENV serotypes, meaning that it is possible to be infected four times. Severe dengue is a leading cause of serious illness and death in some Asian and Latin American countries. It requires management by medical professionals.
What is a serotype of a virus?
Serotypes are groups within a single species of microorganisms, such as bacteria or viruses, which share distinctive surface structures.
How many structural and nonstructural protein are there in the dengue virus?
What is dengue NS1 Elisa?
NS1 tests detect the non-structural protein NS1 of dengue virus. This protein is secreted into the blood during dengue infection. NS1 tests have been developed for use in serum. Most of these tests use synthetically labeled antibodies to detect dengue NS1 protein.
What is serotypes in virus?
A serotype or serovar is a distinct variation within a species of bacteria or virus or among immune cells of different individuals. These microorganisms, viruses, or cells are classified together based on their surface antigens, allowing the epidemiologic classification of organisms to the subspecies level.
How do serotypes differ?
The O antigens are distinguished by their different chemical make-up. The H antigens are distinguished by the protein content of the flagella. Each O and H antigen has a unique code number. Scientists determine the serotype based on the distinct combination of O and H antigens.
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