Table of Contents
Is GFP a fusion protein?
INTRODUCTIONGFP (green fluorescent protein) fusion proteins have been used to address a wide range of questions in individual cells, as well as in tissues of a particular organism. GFP fusion proteins can be transiently or stably expressed.
How do you make fusion proteins?
Naturally occurring fusion genes are most commonly created when a chromosomal translocation replaces the terminal exons of one gene with intact exons from a second gene. This creates a single gene that can be transcribed, spliced, and translated to produce a functional fusion protein.
How many kDa is GFP?
27 kDa
The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27 kDa.
How do you link two proteins together?
The Protein Man Says: Protein cross-linking is the process of binding two or more protein molecules together to facilitate scientific probes on protein-protein interactions. To achieve this effect, specific crosslinking reagents (crosslinkers) are used to chemically join the protein molecules.
How do you make chimeric proteins?
Chimeric proteins can easily be prepared by recombinant means in vitro by fusing the structural genes of the proteins in question in a suitable expression vector. The translational 3′-terminus of the first gene is deleted, as is the promoter of the 5′-terminus of the second structural gene.
What is the maturation time for fluorescent proteins?
We determined an average maturation time of 5.38±0.2 min at normalized fluorescence intensity, FI = 63\% for the S strain (Figure 1A, Table S2) at an average growth rate of 0.75±0.08 1/h and lag-time 52.5±8.3 min. This value is in accordance to other maturation times for GFP obtained in E.
How does red fluorescent protein work?
Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited. Several variants have been developed using directed mutagenesis. The original was isolated from Discosoma, and named DsRed. Others are now available that fluoresce orange, red, and far-red.