Table of Contents
- 1 What is the lipemia index?
- 2 How do you test for lipemia?
- 3 What is serum indices?
- 4 How does serum remove lipemia?
- 5 What causes lipemic?
- 6 How are serum indices measured?
- 7 How do you prevent lipemic blood?
- 8 What is the normal appearance of serum after centrifugation?
- 9 What is the lipemia index used for?
- 10 How does lipemia affect the results of lab tests?
What is the lipemia index?
The HIL Index is widely used by laboratories using chemistry analyzers to identify samples showing possible interference due to hemolysis, icterus, or lipemia at a concentration increased enough to cause an increase or decrease in the analyte concentration by 10\% (1).
How do you test for lipemia?
Lipemia can be detected visually if the concentration of triglycerides in patient sample is over 3.4 mmol/L (32). In the full blood samples, visual detection is very hard and can be observed at much higher concentration of triglycerides (over 11.3 mmol/L) (32).
What is serum indices?
Liquichek Serum Indices is intended for use as part of laboratory interference testing to monitor an instrument’s response in detecting hemolyzed, icteric or lipemic (HIL) samples to help improve detection of pre-analytical errors affecting clinical chemistry testing.
What does Lipaemic mean?
Lipaemia is defined as an abnormally high concentration of lipids in the blood, usually in the form of very low density lipoproteins (VLDLs) or chylomicrons. Characteristically the blood plasma may appear white or milky in colour due to the presence of fat.
How do you prevent lipemia?
One way to avoid grossly lipemic samples is to ask that patients fast for 12 hours before sample collection. If this is impractical, a mechanical-based means of clot detection should be available when samples are grossly lipemic.
How does serum remove lipemia?
Conclusions: High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples.
What causes lipemic?
The most common cause of lipemia is nonfasting, with recent ingestion of lipid-containing meal. More severe lipemia results from a disease condition causing hypertriglyceridemia (eg, diabetes, genetic hyperlipidemia) or recent intravenous infusion of a lipid emulsion.
How are serum indices measured?
The Serum Indices Gen. 2 assay is based on calculations of absorbance measurements of diluted samples at different dichromatic wavelength pairs to provide a semi-quantitative representation of levels of lipemia, hemolysis and icterus present in serum and plasma samples.
Why is it important to measure and report serum indices report?
Serum indices (SI) is a tool which guides laboratory professionals about interferences, increases the quality of the sample, and minimizes aberrant test results. It has been reported that hemolysis accounts for 40\%–70\% of unsuitable specimens sent to the clinical laboratory [5, 6].
What causes lipemic blood?
The most common cause of lipemia is that the patient is not fasting and has eaten close in time to the blood draw. This effect is most dramatic when the patient has consumed a meal with high fat content. However, nonfasting on its own usually does not result in enough lipemia to significantly impact laboratory tests.
How do you prevent lipemic blood?
Please avoid food with high fat content before blood donation. If a blood donor consumes food with high fat content such as oily foods which are fried or deep fried before blood donation it can lead to a transient rise in the triglycerides or cholesterol levels resulting in lipemic blood.
What is the normal appearance of serum after centrifugation?
After centrifugation, what remains is a clear, straw-colored liquid on top of a dark red clot (the clumped blood cells tangled in the fibrin mesh). This straw-colored, acellular liquid is called serum (see Figure 2). FIGURE 2: Serum – the acellular fraction of blood that has been allowed to clot.
What is the lipemia index used for?
A common use of the Lipemia index is to automatically detect significant, visually observable lipemia in patient serum/plasma samples that may cause interference and thus affect clinical results.
Can monoclonal proteins increase lipemia index?
In our study, the Lipemia index was found to be increased due to the presence of a monoclonal protein in 48\% of cases that it triggered. This rate of detection of monoclonal proteins is quite good when considering that the Lipemia index in the absence of visually observable lipemia flag is triggered only in about 0.015\% (24/160000) of CMP samples.
What are lipemic samples?
What Are Lipemic Samples. Lipemic samples are patient specimens that have a higher fat content in them, so their blood is a little more milky, thicker. Whereas, usually when you spin down whole blood, it will look like this. It has yellow, clear serum or plasma on top of the red cells. This is plasma here on top of the red blood cells.
How does lipemia affect the results of lab tests?
This mechanism is probably the most common way in which lipemia affects results of laboratory tests. Lipoprotein particles in the sample can absorb light. The amount of absorbed light is inversely proportional to the wavelength and decreases from 300 to 700 nm, with no specific absorption peaks in between (22).