Table of Contents
What is the function of ROP in pBR322?
Furthermore, pBR322 encodes a functional rop gene that regulates copy number. The Rop protein is involved in stabilizing the interaction between RNA I and RNA II, which in turn prevents the replication of pBR322.
What does ROP mean?
Retinopathy of prematurity (ROP) is an eye disorder caused by abnormal blood vessel growth in the light sensitive part of the eyes (retina) of premature infants. ROP generally affects infants born before week 31 of pregnancy and weighing 2.75 pounds (about 1,250 grams) or less at birth.
What is ROP in a cloning vector?
The gene ‘rop’ present in pBR322 cloning vector, codes for the proteins involved in the replication of the plasmid. So, the correct option is ‘Option B’ .
Is ROP a gene?
In addition to known clinical risk factors such as low birth weight and gestational age, there is a growing body of evidence supporting a genetic basis for ROP.
Is ROP protein a promoter?
One promoter directs synthesis of the primer RNA precursor. We propose that this polypeptide is the rop gene product and that it regulates plasmid DNA replication by modulating the initiation of transcription of the primer RNA precursor.
How is ROP test done?
This test is done inside the neonatal unit or hospital, during a visit by the retina specialist or paediatric ophthalmologist. The pupils of the eye are dilated using drops and the retina is examined with an indirect ophthalmoscope for signs of ROP.
What are the stages of ROP?
Are there different stages of ROP?
- Stage I — Mildly abnormal blood vessel growth.
- Stage II — Moderately abnormal blood vessel growth.
- Stage III — Severely abnormal blood vessel growth.
- Stage IV — Partially detached retina.
- Stage V — Completely detached retina and the end stage of the disease.
What is Rop in pBR322 Class 12?
The different genes present in the vector have different functions. Complete answer: The ‘rop’ gene which is present in the pBR322 codes for the rop protein. Its main function is in decreasing the copy number and so this gene is involved in the replication of the plasmid.
What is the role of ROP protein in a plasmid Mcq?
What is the role of Rop protein in a plasmid? Explanation: This protein which forms a dimer enhances the pairing between RNAI and RNAII so that processing of the primer can be inhibited even at low RNAI concentration. 9.
What is compatibility of plasmid?
When plasmids are compatible, different CBPs bind to each plasmid type, and different NTPases separate the plasmid pairs into the new daughter cells. For high copy plasmids, incompatibility due to partitioning is similar to incompatibility due to having the same replication machinery.
What is an ROP examination?
Definition. Eye exams can show the tissue and blood vessels at the back of the eye. This area is called the retina of the eye. Babies with retinopathy of prematurity (ROP) have problems with blood vessel growth in this area.
Who needs ROP screening?
Infants with a birth weight of 1,500 and 2,000 grams or a gestational age of more than 30 weeks should be screened if other health troubles put them at high risk for ROP. The first exam should occur four to nine weeks after birth, depending on how premature the baby is.
What is the meaning of “ROP codes”?
It is not implied as “rop codes” as is a phrase. Rop is the name of a gene that codes for the proteins involved in the regulation of replication of the plasmid. Rop stands for regulator of origin of plasmid. Rop proteins are involved in maintenance of copy number of plasmid DNAs by mechanisms as also explained by other members.
What is the function of the rop gene?
Rop is a gene which codes for a small protein responsible for keeping the copy number of ColIEI and related bacterial plasmid low in E.coli Structurally, Rop is a homodimeric four-helix bundle protein formed by the antiparallel interaction of two helix-turn-helix monomers. The Rop protein’s structure has been solved to high resolution.
What is the function of ROP in E coli?
Rop is a gene and also known as repressor of primer. Rop is a gene which codes for a small protein responsible for keeping the copy number of ColIEI and related bacterial plasmid low in E.coli Structurally, Rop is a homodimeric four-helix bundle protein formed by the antiparallel interaction of two helix-turn-helix monomers.
What is the function of the rop gene in PBR322?
One potential explanation for this exclusion effect is the presence of a functional rop gene in pBR322. The rop gene encodes for a protein that stabilizes the interaction between RNA I and RNA II, thus inhibiting the RNA II from carrying out its function as a primer to begin DNA replication.