Table of Contents
What is the difference between HEK293 and HEK293t?
The key difference between HEK293 and HEK293t is that HEK293 is an immortalized cell line derived from the embryonic human kidney that is transfected with sheared human adenovirus type 5DNA while HEK293t is a daughter cell line derived from HEK293 original cell line that is transfected with a plasmid vector carrying …
Is HEK293 normal cell line?
HEK293 cell line was frequently used as non- or low tumorigenic for testing oncogenic properties of cancer-associated genes. Thus, HEK 293T cell line cannot be used as a normal cells (from “HEK293 cell line in cell biology and cancer research: origin, phenotype, karyotype, tumorigenicity”, manuscript in preparation).
What cells are used to grow adenovirus?
These cell lines are based on human primary amniocytes, human embryonic lung HEL 299 cells or HeLa with the incorporated E1A region driven by an alternate promoter (as detailed in Table 1). Unfortunately, the HeLa cell line is not allowed for commercial use because of its high tumorigenicity.
What is a producer cell?
The producer cell line is a key component of any CGMP manufacturing campaign for a gene therapy vector. The efficiency with which our proprietary cell lines can propagate virus allows us to generate high yields of your product with the highest levels of purity.
What is the 293 in HEK293?
What Is HEK293? HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5). The ‘293’ refers to the fact that it was Graham’s 293rd experiment.
What does HEK293 mean?
Human embryonic kidney 293 cells
Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific immortalised cell line derived from an aborted fetus or human embryonic kidney cells grown in tissue culture taken from a female fetus in 1973.
Is HEK293 cancerous?
Meanwhile, we also find that high-passage HEK 293 can be employed as a highly malignant tumor model as its tumorigenicity increases significantly.
How does adenovirus enter the cell?
The virus enters the cell in a clathrin-coated vesicle and is transported to endosomes, where acidification results in partial disassembly of the capsid; the altered virion escapes into the cytoplasm and is transported to the nucleus, where replication occurs (54).
How does the adenovirus leave a cell to infect other cells?
Almost all serotypes adenoviruses use the coxsakie- and adenovirus- receptor (CAR) as the primary receptor on the host cell. Viral replication and assembly occurs in the nucleus of infected cells and mature viruses leave the host cell via disintegration.
What is a packaging cell line?
Packaging Lines Currently, the most efficient gene transfer methodologies harness the capacity of engineered viruses, such as retroviruses, to bypass natural cellular barriers to exogenous nucleic acid uptake.
What is a stable producer cell line?
The stable producer cell line, called clone 92, was derived by stable transfection from a packaging cell line with a plasmid encoding the transgene GFP. The packaging cell line expresses all the other necessary components to produce LV upon induction with cumate and doxycycline.