Sage-Tips

How do you make agarose gel for DNA?

1. Preparation of the Gel

1. Weigh out the appropriate mass of agarose into an Erlenmeyer flask. Agarose gels are prepared using a w/v percentage solution.
2. Add running buffer to the agarose-containing flask. Swirl to mix.
3. Melt the agarose/buffer mixture.
4. Add ethidium bromide (EtBr) to a concentration of 0.5 μg/ml.

How do you boil agarose gel?

Microwave for 1-3 min until the agarose is completely dissolved. It is best to heat 30 seconds, stop and swirl towards a boil. Do not over boil and be careful stirring as eruptive boiling can occur. Placing cling film or a lid over the top can help (do not secure the lid, just place it on top).

How do you melt agarose gel?

To melt the agarose in solutions of less than 2\%, heat the slurry in microwave oven on high power setting until it starts to boil. Allow the solution to boil for 1 min or until the solution is clear and all particles are dissolved. Remove the flask from the microwave oven, and gently swirl to mix the agarose solution.

How do you make a 2 agarose gel?

2.0\% Agarose

1. Add 4.0 g agarose (electrophoresis grade) to 200 ml 1X TBE electrophoresis buffer in a 600 ml beaker or Erlenmeyer flask.
2. Stir to suspend agarose.
3. Cover beaker with aluminum foil, and heat in boiling-water bath (double boiler) or on hot plate until all agarose is dissolved (approximately 10 minutes).

What is the tape for on the gel casting tray?

Note that the tape is folded around the ends of the gel tray. This stops the Agarose from leaking out the ends.

How do you melt agarose gel without a microwave?

Product: HyAgarose™ LM Agarose, Low Melting 4) If use boiling water bath: • To melt agarose, simply heat the slurry in a boiling water bath, bring the solution to a boil and allow it to boil for 5-10 minutes stirring continuously, until agarose dissolves completely.

How do you dissolve agarose powder?

Agarose is available as a white powder which is insoluble in cold water and dissolves (slowly soluble) in hot (near-boiling) water. A solution forms a stiff gel upon cooling.

How can DNA fragments be Visualised in gel electrophoresis?

In agarose gel electrophoresis, separated DNA fragments can be visualised with the help of ethidium bromide in UV radiation.