Table of Contents
What organism is green fluorescent protein found in?
jellyfish Aequorea victoria
Green fluorescent protein (GFP) is a protein produced by the jellyfish Aequorea victoria, that emits bioluminescence in the green zone of the visible spectrum. The GFP gene has been cloned and is used in molecular biology as a marker.
What are some uses of the GFP protein?
GFP is used in research across a vast array of biological disciplines and scientists employ GFP for a wide number of functions, including: tagging genes for elucidating their expression or localization profiles, acting as a biosensor or cell marker, studying protein-protein interactions, visualizing promoter activity.
What does it mean to say that green fluorescent protein is fluorescent?
GFP
Green fluorescent protein: Abbreviated GFP. A protein that glows green under fluorescent light. Found naturally in the jellyfish Aequorea victoria, GFP fluoresces green when exposed to blue light. It has a sequence of three amino acids (serine-tyrosine-glycine) which is responsible for its fluorescence.
Are there different types of GFP?
Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered.
Why are fluorescent proteins important?
The function of the fluorescent protein is to act as a bioluminescence resonance energy transfer (BRET) acceptor that converts the otherwise blue emission of the bioluminescent protein into a longer wavelength green emission.
What are examples of fluorescent light?
A CFL light bulb that works through radiation is an example of a fluorescent light. A bright, vivid, colorful pink couch is an example of a fluorescent couch.
What is called fluorescent?
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, than the absorbed radiation.
How many different types of fluorescent proteins are there?
Table 1 – Fluorescent Protein Properties
| Protein (Acronym) | Excitation Maximum (nm) | Emission Maximum (nm) |
|---|---|---|
| Azurite | 384 | 450 |
| mTagBFP | 399 | 456 |
| Cyan Fluorescent Proteins | ||
| ECFP | 439 | 476 |
Is red fluorescent protein hydrophobic?
To our knowledge, pHuji is the first confirmed pH sensitive red fluorescent protein which is compatible with hydrophobic resin embedding.
What are fluorescent bulbs?
A fluorescent lamp tube is filled with a mix of argon, xenon, neon, or krypton, and mercury vapor. The pressure inside the lamp is around 0.3\% of atmospheric pressure. The partial pressure of the mercury vapor alone is about 0.8 Pa (8 millionths of atmospheric pressure), in a T12 40-watt lamp.
What is the function of green fluorescent protein?
Concept 34 Genes can be moved between species. Green fluorescent protein (GFP) is a protein that causes the Aequorea victoria jellyfish to glow. The protein is coded for by a single gene. The GFP gene can be inserted downstream of the promoter of a gene in another organism. RNA polymerase binds to promoter regions to initiate transcription.
What does green fluorescent protein mean?
The green fluorescent protein is a protein composed of 238 amino acid residues that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria.
How is green fluorescent protein used in research?
Green fluorescent protein ( GFP ) and related fluorescent proteins have become an integral part of molecular biology for a variety of uses including visualizing protein localization and as intracellular sensors (i.e. pH, Ca2+, redox environment). The widespread use of GFP is owed to its remarkable self-catalyzed chromophore formation.
What is GREEN FLUORESCENCE PROTEIN?
Green Fluorescent Protein (GFP) Green Fluorescent Protein (GFP) is a protein produced by a jellyfish Aequorea victoria ; which produces glowing points of light around the margin of it’s umbrella. The light arises from yellow tissue masses that each consist of about 6000-7000 photogenic cells.