Table of Contents
What is the principle of glucose test?
The enzyme glucose oxidase reacts with glucose, water, and oxygen to form gluconic acid and hydrogen peroxide. The hydrogen peroxide can then be used to oxidize a chromogen or the consumption of oxygen measured to estimate the amount of glucose present.
What is the significance of glucose tolerance test?
Clinical Significance The glucose tolerance test is given to determine how quickly glucose is cleared from the blood. The test is used to test for diabetes, insulin resistance, impaired beta cell function, reactive hypoglycemia, acromegaly, and other disorders of carbohydrate metabolism.
What is the principle of glucose oxidase?
Principle: Glucose oxidase is an enzyme extracted from the growth medium of Aspergillus niger. Glucose oxidase catalyse the oxidation of Beta D- glucose present in the plasma to D glucono -1 ,5 – lactone with the formation of hydrogen peroxide; the lactone is then slowly hydrolysed to D-gluconic acid.
What is glucose determination?
Blood glucose determination is one of the most common clinical diagnostic tests. Often, blood is collected in a field station and analysis is carried out in a remote laboratory. Because blood cells can continue to metabolize glucose, the time of determination of blood glucose after drawing the blood is important.
What is administration of insulin?
Conventional insulin administration involves subcutaneous injection with syringes marked in insulin units. There may be differences in the way units are indicated, depending on the size of the syringe and the manufacturer. Insulin syringes are manufactured with 0.3-, 0.5-, 1-, and 2-ml capacities.
What is the principle of God-Pod method?
analyzer): Glucose oxidase enzyme (GOD) oxidizes the specific substrate beta-D-glucose to gluconic acid and hydrogen peroxide is liberated. Peroxidase enzyme acts on hydrogen peroxide to liberate nascent oxygen (O). Nascent oxygen then couples with 4-amino- antipyrine and phenol to form red quinoneimine dye.
What is the principle of glucose oxidase method?
Other uses of glucose oxidase include the removal of oxygen from food packaging and removal of D-glucose from egg white to prevent browning. PRINCIPLE: Glucose oxidase catalyses the oxidation of β-D-glucose to D-glucono- δ-lactone with the concurrent release of hydrogen peroxide (1).
What is the assay range for glucose?
A blood glucose test is a blood test that screens for diabetes by measuring the level of glucose (sugar) in a person’s blood. Normal blood glucose level (while fasting) range within 70 to 99 mg/dL (3.9 to 5.5 mmol/L). Higher ranges could indicate pre-diabetes or diabetes.
Why insulin is given subcutaneously?
The preferred tissue space for insulin injection is the subcutaneous layer, which is the fat layer just below the dermis and above the muscle1; it offers slow, stable and predictable absorption, whatever the fat tissue depth2. Stable and predictable absorption of insulin will support optimal blood glucose control.
Why is insulin not administered orally?
Insulin cannot be taken by mouth because it is digestible. Oral insulin would be obliterated in the stomach, long before it reached the bloodstream where it is needed. Once injected, it starts to work and is used up in a matter of hours.
What is the principle of glucose estimation by God-Pod method?
The GOD-POD method is linear (up to 500 mg/dl), sensitive (detection limit 0.3 mg/dl), simple (requires 10 microlitre of sample to be incubated for 30 minutes with single reagent at room temperature) and requires simple instrumentation (the absorbance to be read between 505 nm to 550 nm).
What is the route for insulin injection?
Insulin is injected subcutaneously, which means into the fat layer under the skin. In this type of injection, a short needle is used to inject insulin into the fatty layer between the skin and the muscle. Insulin should be injected into the fatty tissue just below your skin.