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What is high power field in urinalysis?
From Wikipedia, the free encyclopedia. A high-power field (HPF), when used in relation to microscopy, references the field of view under the maximum magnification power of the objective being used. Often, this represents a 400-fold magnification when referenced in scientific papers.
How many UL are in HPF?
One HPF of microscopy for urinary sediment is theoretically equivalent to 0.45µL of non-centrifuged urine sample. However, in fact, there is discrepancy between theory and practice for some reasons. In this study, we examined the variance using KOVA slide.
What magnification is high power?
| Magnification | Total Magnification | |
|---|---|---|
| Scanning | 4x | 40x |
| Low Power | 10x | 100x |
| High Power | 40x | 400x |
| Oil Immersion | 100x | 1000x |
What is a high power field pathology?
A high power field (HPF) count is used to quantify positivity in a tissue section and may influence the diagnosis or staging of the patient. A pathologist performs HPF counts by scanning and estimating the number of positive cells.
What is HPF count?
What is uL unit?
Microlitre or microliter (µl), a unit of volume.
How do you calculate high power magnification?
To calculate the total magnification of the compound light microscope multiply the magnification power of the ocular lens by the power of the objective lens. For instance, a 10x ocular and a 40x objective would have a 400x total magnification. The highest total magnification for a compound light microscope is 1000x.
How do you calculate high power field of view?
Field of View = Field Number (FN) ÷ Objective Magnification You will have to multiply the eyepiece magnification by the objective magnification to find the total magnification before dividing the field number. For instance, if your eyepiece reads 10X/22, and the magnification of your objective lens is 40.
How many mm2 is HPF?
The most recent revision of the American Joint Committee on Cancer melanoma staging system now incorporates MR and specifies that MR should be reported as mitoses per mm2, with a conversion factor of 1 mm2 equaling 4 hpf.
What is iU L and U L?
iU/L is international units per litre, and U/L is just units.
How does a refractometer measure specific gravity in urine?
A refractometer measures the change in direction of a light path (refraction) based upon particle concentration and size in a fluid. Larger particles such as glucose and albumin will alter refraction to a greater degree. The urine dipstick measurement of specific gravity is an approximation that is most sensitive to cationic concentration in urine.
What is urine analysis with microscopy dipstick methodology?
Urine Analysis with Microscopy Dipstick Methodology •Paper tabs impregnated with chemical reagents •Reagents are chromogenic •Reagents are timed developed •Some rxnsare highly specific •Other are sensitive to the presence of interfering substances or extremes of pH •Rapid, semiquantitative assessment of urinary characteristics
What is the specific gravity of urine in end stage renal disease?
In end-stage renal disease, sp gr tends to become 1.007 to 1.010. Any urine having a specific gravity over 1.035 is either contaminated, contains very high levels of glucose, or the patient may have recently received high density radiopaque dyes intravenously for radiographic studies or low molecular weight dextran solutions.
What are the components of urine analysis?
Urine Analysis •Appearance or color •Specific gravity •pH •Leukocyte esterase •Nitrites •Urobilinogen •Bilirubin •Glucose •Ketones •Protein •Blood •Microscopic examination Proper Specimen Collection •Teach every patient how to collect proper specimen 1. Clean-catch midstream 2.