Table of Contents
- 1 What does Ortho Tuluidine reagent contain?
- 2 Is toluidine toxic?
- 3 What is the Iupac name of Orthotolidine?
- 4 What is difference between toluene and toluidine?
- 5 Is p-toluidine organic?
- 6 What is Ortho Tolidine?
- 7 What are the precautions to be taken while using laboratory reagents?
- 8 What are the rules and regulations of a laboratory?
What does Ortho Tuluidine reagent contain?
95-53-4; C7H9N; 1-amino-2- methylbenzene, 2-aminotoluene, o-methylaniline), a synthetic chemical described as having an “aromatic” odour, exists at ambient temperature as a light yellow liquid that rapidly darkens on exposure to air and light. o-Toluidine has a boiling point of 200°C, a melting point of !
What is toluidine used for?
ortho-Toluidine is used as an intermediate in the synthesis of the large-volume herbicides, metolachlor and acetochlor, in the manufacture of more than 90 dyes and pigments (e.g. acid-fast dyestuffs, azo pigment dyes, triarylmethane dyes, sulfur dyes, and indigo compounds), and as an intermediate for synthetic rubber …
Is toluidine toxic?
o-Toluidine is primarily used in the manufacture of dyes. o-Toluidine is highly toxic to humans when absorbed through the skin, inhaled as vapor, or swallowed. Acute (short-term) exposure of humans to o- toluidine affects the blood (i.e., methemoglobinemia), with clinical signs of central nervous system depression.
What is o-Toluidine method?
An o-Toluidine Method for Body-Fluid Glucose Determination o-Toluidine, 6\% (v/v) in glacial acetic acid, is used to determine glucose in biologic material after deproteinization with 3\% (w/v) trichloracetic acid. The development of the procedure is discussed, as is the specificity of the method for glucose.
What is the Iupac name of Orthotolidine?
Tolidine
| Names | |
|---|---|
| Preferred IUPAC name 3,3′-Dimethyl-[1,1′-biphenyl]-4,4′-diamine | |
| Other names o-Tolidine; Orthotolidine; Diaminoditolyl; Diaminotolyl; Bianisidine; Tolidine blue; 3,3′-Dimethylbenzidine; 4,4′-Bi-o-toluidine | |
| Identifiers | |
| CAS Number | 119-93-7 |
What is the function of Ortho toluidine in glucose test?
o-Toluidine, 6\% (v/v) in glacial acetic acid, is used to determine glucose in biologic material after deproteinization with 3\% (w/v) trichloracetic acid. A stable green color develops after heating at 100° for 10 min., and the absorbance is determined at 630 or 635 mµ.
What is difference between toluene and toluidine?
is that toluene is (organic compound) a colourless, inflammable liquid hydrocarbon, methylbenzene, ch3c6h5, used as a solvent, in high-octane fuels and in the production of many chemical compounds while toluidine is (organic compound) any of the three isomeric aromatic amines derived from toluene; they are used in the …
What functional group is p-toluidine?
methyl group
P-toluidine is an aminotoluene in which the amino substituent is para to the methyl group.
Is p-toluidine organic?
There are three isomers of toluidine, which are organic compounds. These isomers are o-toluidine, m-toluidine, and p-toluidine, with the prefixed letter abbreviating, respectively, ortho; meta; and para.
Is p-toluidine soluble in ether?
This compound is soluble in alcohol, ether, dilute acids, and water. It is soluble in acetone, benzene, diethyl ether, ethanol, and water. p-Toluidine also degrades like the other isomers of toluidine, with a half-life in atmosphere of about 3 h and volatization from wet soil and aqueous environments.
What is Ortho Tolidine?
o-Tolidine is a diacidic dye which is sparingly soluble in water and easily soluble in ether and alcohol. This forms the basis for detection of chlorine, nitrates and hydrogen peroxide in air or water. It turns yellow on reacting with free chlorine. o-Tolidine easily forms acid salts.
What is glucose oxidase method?
The enzyme glucose oxidase reacts with glucose, water, and oxygen to form gluconic acid and hydrogen peroxide. The hydrogen peroxide can then be used to oxidize a chromogen or the consumption of oxygen measured to estimate the amount of glucose present.
What are the precautions to be taken while using laboratory reagents?
Maintain the minimum stock of laboratory reagents/chemicals to prevent any delay in the analysis. Always assure the intactness of the reagent bottle before use. Ensure the expiry date of a particular reagent before using it. Do not use any reagents after the expiry date as mentioned on the label of the bottle/container.
What are the laboratory precautions and safety procedures?
LABORATORY PRECAUTIONS AND SAFETY PROCEDURES . LAB SAFETY . 1. No food or drink is allowed in lab unless food or drinks are provided as a part of the lab. Even though lab tables and counters are wiped down before each lab set up, as a result of some laboratory exercises, chemical residues may be present on the tables.
What are the rules and regulations of a laboratory?
General lab safety rules The following are rules that relate to almost every laboratory and should be included in most safety policies. They cover what you should know in the event of an emergency, proper signage, safety equipment, safely using laboratory equipment, and basic common-sense rules.
What precautions should I take when working with electrophoresis equipment?
Take the following precautions when working with electrophoresis equipment: Ensure all switches and indicators are in proper working condition and that power cords and leads are undamaged and properly insulated. Label equipment with the warning: “Danger Electrical Hazard.”