Table of Contents
What do fluorescent proteins do?
The function of the fluorescent protein is to act as a bioluminescence resonance energy transfer (BRET) acceptor that converts the otherwise blue emission of the bioluminescent protein into a longer wavelength green emission.
What does green fluorescent protein GFP?
Green fluorescent protein (GFP) is a protein produced by the jellyfish Aequorea victoria, that emits bioluminescence in the green zone of the visible spectrum. The GFP gene has been cloned and is used in molecular biology as a marker.
How long does GFP last?
The half-life of the green fluorescent protein (GFP) was determined biochemically in cultured mouse LA-9 cells. The wild-type protein was found to be stable with a half-life of ~26 h, but could be destabilized by the addition of putative proteolytic signal sequences derived from proteins with shorter half-lives.
How do fluorescent markers work?
Fluorescent markers give the ability to investigate proteins in their biological environment. When light of a certain wavelength is directed at the molecule’s chromophore, a photon is absorbed and excites an electron to a higher energy state. Multiple fluorescent markers can be used to stain different parts of cell.
Is green fluorescent protein safe?
These data indicate that GFP is a low allergenicity risk and provide preliminary indications that GFP is not likely to represent a health risk.
How do fluorescent materials work?
Fluorescent materials produce light instantly, when the atoms inside them absorb energy and become “excited.” When the atoms return to normal, in as little as a hundred thousandth of a second, they give out the energy that excited them as tiny particles of light called photons.
What is a fluorescent body?
A bruise raised on the body of a sexual partner by very hard kissing / biting / sucking. spelled also hickey.
How stable is GFP?
After 10 min of contact, GFP exhibited the greatest stability in phosphate buffered solutions (pH 7) for the range of 20- 100 ppm of available chlorine, equivalent to the range of 40- 70 ppm in chlorinated WFI solution, (pH 11 ( 0.23) and to the range of 10-50 ppm in phosphate buffer (pH 6.86 ( 0.21).