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How are HEK 293 cells harvested?
HEK 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex van der Eb’s laboratory in Leiden, the Netherlands. The cells were obtained from a single, aborted or miscarried fetus, the precise origin of which is unclear.
How can a cell line be obtained from a cultured cell?
Cells can be isolated from solid tissues by digesting the extracellular matrix using enzymes such as collagenase, trypsin, or pronase, before agitating the tissue to release the cells into suspension. Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture.
How do you transfect HEK293 cells?
The protocol for a 24-well transfection reaction with HEK293 cells is here:
- Plate 10,000-15,000 HEK293 cells per well in 0.5 ml of complete growth medium 12-24 hours prior to transfection.
- Wash with 1xPBS and add 0.5 ml of fresh growth medium.
Where do HEK 293 cells come from?
HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.
What does the 293 in HEK293 stand for?
What Is HEK293? HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5). The ‘293’ refers to the fact that it was Graham’s 293rd experiment.
How are cells grown in a lab?
There are 2 ways of culturing cells in the laboratory for in vitro experiments. The first is a liquid medium culture called suspension culture done in cell culture flasks, and the second is a semi-solid medium of culturing done in cell culture plates. which is added to the already nutrient-rich culture media.
How do cells grow in culture?
Essentially, cell culture involves the distribution of cells in an artificial environment (in vitro) which is composed of the necessary nutrients, ideal temperature, gases, pH and humidity to allow the cells to grow and proliferate. In vivo – When the study involves living biological entities within the organism.
What is cell line HEK293?
HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. The transformation resulted in the incorporation of approximately 4.5 kilobases from the viral genome into human chromosome 19 of the HEK cells.
What are human embryonic kidney cells used for?
The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production.
What is the best transfection reagent for HEK293?
Conclusion: Lipofectamine 3000 is the best choice for transfection of CHO-K1 and HEK293 with pCDH while Turbofect is preferably used in transfecting these cell lines with pEGFP-N1 (Tab.
What is the meaning of HEK 293 cells?
HEK 293 cells. HEK 293 cells grown for several days in standard tissue culture medium. Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture.
Can HEK293 cells be used to develop new drugs?
Several other companies, such as Moderna and Pfizer, have used HEK293 to develop “pseudoviruses” to test their drugs. Vaccines against Ebola and tuberculosis, as well as gene therapies, have also been created with HEK293 cells, said Graham.
What is HEK 293 and Perper C6?
PER C6 & HEK 293. HEK (human embryonic kidney) 293 – the number of aborted fetal experiments prior to establishing the cell line. HEK 293 is used to deliver the lentivirus gene in Dr Yamanaka’s adult skin cell reprogramming, as a cell line and reagent for testing many research products and in the drug Xigris.
What is the function of the 293T cell line?
An important variant of this cell line is the 293T cell line. It contains the SV40 large T-antigen that allows for episomal replication of transfected plasmids containing the SV40 origin of replication. This allows for amplification of transfected plasmids and extended temporal expression of desired gene products.