Table of Contents
What is bacterial genome sequencing?
Bacterial whole genome sequencing (WGS) is becoming a widely-used technique in research, clinical diagnostic, and public health laboratories. It enables high resolution characterization of bacterial pathogens in terms of properties that include antibiotic resistance, molecular epidemiology, and virulence.
What is meant by genome sequencing projects?
Genome projects are scientific endeavours that ultimately aim to determine the complete genome sequence of an organism (be it an animal, a plant, a fungus, a bacterium, an archaean, a protist or a virus) and to annotate protein-coding genes and other important genome-encoded features.
What is Microbial Genome Project?
The DOE began a Microbial Genome Program in late 1994 to sequence the genomes of some bacteria. The program strives to better understand the bountiful microbial resources on Earth. Organziations around the world have worked to decipher the genomes of dozens of microorganisms.
What is the purpose of the genome project?
The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome.
What are the steps of Sanger sequencing?
There are three main steps to Sanger sequencing.
- DNA Sequence For Chain Termination PCR. The DNA sequence of interest is used as a template for a special type of PCR called chain-termination PCR.
- Size Separation by Gel Electrophoresis.
- Gel Analysis & Determination of DNA Sequence.
How long does it take to sequence a bacterial genome?
Table 1
| Method | Single-Molecule Real-Time Sequencing (Pacific Bio) | Sequencing by Synthesis (Illumina) |
|---|---|---|
| Read length | 2,900 bp | 50 to 250 bp |
| Accuracy | 99\% | 98\% |
| Reads per run | 35–75 thousand | up to 3 billion |
| Time per run | 30 min to 2 h | 1 to 10 days, |
What are the basic steps of genome project?
The shotgun phase of the Human Genome Project itself consisted of three steps: Obtaining a DNA clone to sequence. Sequencing the DNA clone. Assembling sequence data from multiple clones to determine overlap and establish a contiguous sequence.
Who coordinated the Human Genome project?
“The US side of the Human Genome Project was initially led by James Watson. The US side of the Human Genome Project was initially led by James Watson (one half of Crick and Watson, who discovered the structure of DNA?), and later by Francis Collins.
What is DNA Sanger sequencing?
Sanger sequencing, also known as the “chain termination method”, is a method for determining the nucleotide sequence of DNA. The method was developed by two time Nobel Laureate Frederick Sanger and his colleagues in 1977, hence the name the Sanger Sequence.