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What does the 293 in HEK 293 stand for?
What Is HEK293? HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5). The ‘293’ refers to the fact that it was Graham’s 293rd experiment.
Is HEK 293 cancerous?
Meanwhile, we also find that high-passage HEK 293 can be employed as a highly malignant tumor model as its tumorigenicity increases significantly.
Why are HEK 293 cells used?
HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy as well as safety testing for a vast array of chemicals.
Are HEK 293 cells immortal?
HEK 293 immortal cell line that is comparatively easy to handle. An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division.
How was HEK293 immortalized?
The HEK293 cell line was originally immortalized by the random integration of viral genomic DNA of adenovirus 518, which includes the E1A and E1B genes.
What are 293 cells?
Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific immortalised cell line derived from an aborted fetus or human embryonic kidney cells grown in tissue culture taken from a female fetus in 1973.
Are HEK293 immortal?
HEK293 is embryonic kidney immortalized cell line, which represents the epithelial morphology and possibility to form tumors in nude mice. HEK 293 cells are popular for their ease of growth and transfection, making them a common cell culture in cancer research.
Are HEK293 normal cells?
Thus, HEK293 is not a model for normal human cells; these cells are immortalized already by known oncogene but not malignant yet.
What is the HEK293 cell line?
HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. The transformation resulted in the incorporation of approximately 4.5 kilobases from the viral genome into human chromosome 19 of the HEK cells.
What does HEK mean?
HEK
| Acronym | Definition |
|---|---|
| HEK | Human Embryonic Kidney Cells |
| HEK | Halo Editing Kit (gaming) |
| HEK | Human Epidermal Keratinocyte |
| HEK | Human Embryo Kinase (cell biology) |