Table of Contents
What vaccines is WI 38 used in?
Vaccines for the following virus-based diseases were developed using WI-38: poliomyelitis, measles, mumps, rubella, varicella (chicken pox), herpes zoster,6 adenovirus, rabies and Hepatitis A.
How are MRC-5 cells obtained?
Most of these cell lines (including MRC-5 cells and 293 cells) were derived from tissue samples taken from foetuses aborted in the 1960s and 1970s and the cells have been grown in laboratories all over the world since then.”
What is the WI-38 cell line?
WI-38 is a diploid human cell line composed of fibroblasts derived from lung tissue of a 3-month-gestation female fetus. The fetus came from the elective abortion of a Swedish woman in 1962, and was used without her knowledge or permission.
How are cell lines developed?
The simplest way to create a new cell line is to modify an existing one, a common strategy when an established line already comes close to meeting the requirements. Cells optimized to grow particular viruses or maximize recombinant protein production often come from such modifications.
Is MRC-5 immortalized?
Stable expression of hTERT in MRC-5hTERT cells was sufficient to bypass senescence and extend the proliferative life span of the parental MRC-5 cell line beyond one year in culture (Figure 1B). Therefore, the MRC-5hTERT cells can be designated as immortalized, as has been previously reported (13,27).
Where does WI 38 come from?
Is WI-38 immortalized?
Immortalization of human WI38 cells is associated with differential activation of the c-myc origins. J Cell Biochem.
Is MRC-5 a stem cell?
MRC-5 is the most common human diploid cell line used in production of viral vaccines; mesenchymal stem cells (MSCs) is a type of adult multipotent stem cells. Both cell types share the same fibroblast-like morphology and maintain a normal diploid karyotype over long in vitro expansion.
What is the purpose of cell lines?
Cell lines have revolutionized scientific research and are being used in vaccine production, testing drug metabolism and cytotoxicity, antibody production, study of gene function, generation of artificial tissues (e.g., artificial skin) and synthesis of biological compounds e.g., therapeutic proteins.
What is the difference between cell line and cell strain?
The main difference between cell line and cell strain is that cell line is the first subculture of a cell population of a primary culture whereas cell strain is a subpopulation of a cell line positively selected from the culture after undergoing cloning or some other method.
What is HEK293?
What Is HEK293? HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5). The ‘293’ refers to the fact that it was Graham’s 293rd experiment.
What is a MRC-5 cell?
MRC-5 cells are human fetal lung fibroblast cells. MRC-5 cells were derived from embryonic lung tissue of 14-week-old Caucasian male fetus in 1966. These diploid cells have been used extensively in vaccine production and other research.