Sage-Tips

What is the sequence of the complementary mRNA Strand?

Complementary bases are: adenine (A) and thymine (T), and cytosine (C) and guanine (G). So if one strand of DNA reads A-C-G-C-T-A, then the complementary strand is T-G-C-G-A-T. You can find the sequence of the mRNA transcript in the same way, by using the complements of the bases shown in the DNA sequence.

How do you determine the amino acid sequence of a gene?

How to: Find transcript sequences for a gene

1. Search the Gene database with the gene name, symbol.
2. Click on the desired gene.
3. Click on Reference Sequences in the Table of Contents at the upper right of the gene record.

What will be the sequence of mRNA?

Codons in an mRNA are read during translation, beginning with a start codon and continuing until a stop codon is reached. mRNA codons are read from 5′ to 3′ , and they specify the order of amino acids in a protein from N-terminus (methionine) to C-terminus.

How many amino acids are coded by the mRNA sequence -?

The three-letter nature of codons means that the four nucleotides found in mRNA — A, U, G, and C — can produce a total of 64 different combinations. Of these 64 codons, 61 represent amino acids, and the remaining three represent stop signals, which trigger the end of protein synthesis.

Where can I find the sequence of a gene?

To find the gene coding sequence, look at the Genomic regions, transcripts, and products section or the NCBI Reference Sequences (RefSeq) section of the Gene record: Clicking on the GenBank link displays the GenBank record in the Nucleotide database.

What is the sequence of the DNA coding strand?

When referring to DNA transcription, the coding strand (or informational strand) is the DNA strand whose base sequence is identical to the base sequence of the RNA transcript produced (although with thymine replaced by uracil). It is this strand which contains codons, while the non-coding strand contains anticodons.

How are amino acids coded in mRNA?

Each group of three bases in mRNA constitutes a codon, and each codon specifies a particular amino acid (hence, it is a triplet code). The mRNA sequence is thus used as a template to assemble—in order—the chain of amino acids that form a protein. Multiple codons can code for the same amino acid.

What is the corresponding sequence of bases in mRNA?

If the sequence of bases in a section of DNA is ATCCGATT, what is the corresponding sequence of bases in mRNA? However, mRNA does not consist of the same four bases as DNA. While DNA has the ATCG nitrogenous bases, RNA replaces thymine with uracil, making its bases AUCG.

What is the complementary DNA strand to the template strand?

The coding DNA strand, which is complementary to the template strand, is 5’ ATGGCAACCCAGGGTAGTTTG 3’ c. The sequence of the mRNA is 5’ AUGGCAACCCAGGGUAGUUUG 3’

Which DNA fragments migrate more quickly through the sequencing gel?

They have a hydrogen at the 3′ carbon of the sugar. DNA fragments that are 600 bp long will migrate more quickly through a sequencing gel than fragments that are 150 bp long. False. Which of the following statements about manual Sanger sequencing is true?

Why do regulatory sequences lie upstream of the gene?

They typically lie upstream of the gene and can have regulatory elements several kilobases away from the transcriptional start site. In eukaryotes, the transcriptional complex can cause the DNA to bend back on itself, which allows for placement of regulatory sequences far from the actual site of transcription.