Table of Contents
What is responsible for color change in dische test?
The Dische’s Test will detect the deoxyribose of DNA and will not interact with the ribose in RNA. The amount of blue corresponds to the amount of DNA in solution. The diphenylamine compound of the Dische’s test interacts with the deoxyribose of DNA to yield a blue coloration.
What is the color produced upon addition of diphenylamine reagent?
The blue color that has been obtained by the action of nitrosamines, nitrates, nitrites, and aliphatic nitro- compounds on diphenylamine in sulfuric acid has been regarded [1]1 as a specific test for these sub- stances in the absence of various oxidizing agents that also produce a blue color.
Why is diphenylamine used as an indicator?
Diphenylamne is used as an indicator because it shows a very clear color change from green to violet when end point of the titration is reached. Usually phosphoric acid is added to the Fe2+ solution (ferrous ammonium sulfate) if that is the reductant that is being titrated, so that the Fe3+ product may be stabilized.
Is diphenylamine toxic?
On the basis of mammalian toxicity data Diphenylamine is classified as toxic. Therefore, an assessment of secondary poisoning is carried out.
How does the diphenylamine test work?
The (Dische) Diphenylamine Test is used for determining the presence of nucleic acids. The presence of DNA will turn a clear solution blue. The more DNA present the darker the color. Another nucleic acid, RNA, will turn green.
What is the chemical reaction involved between diphenylamine and DNA?
The deoxyribose in DNA in the presence of acid forms β-hydroxylevulinaldehyde which reacts with diphenylamine to give a blue colour with a sharp absorption maximum at 595nm. In DNA, only the deoxyribose of the purine nucleotides react, so that the value obtained represents half of the total deoxyribose present.
How does diphenylamine reagent differentiate RNA and DNA?
Which type of indicator is diphenylamine?
Redox indicator Many diphenylamine derivatives are used as redox indicators that are particularly useful in alkaline redox titrations.
Is diphenylamine a carcinogen?
* While Diphenylamine has been tested, further testing is required to assess its potential to cause cancer. * Diphenylamine may damage the developing fetus. * Diphenylamine may damage the kidneys (polycystic kidneys) and may affect the bladder. * Overexposure may affect the liver.
What is diphenylamine common name?
Diphenylamine
| Names | |
|---|---|
| Preferred IUPAC name N-Phenylaniline | |
| Other names (Diphenyl)amine Diphenylamine (deprecated) Diphenylazane N-Phenylbenzenamine Anilinobenzene (Phenylamino)benzene N,N-Diphenylamine C.I. 10355 Phenylbenzenamine | |
| Identifiers | |
| CAS Number | 122-39-4 |
Why does diphenylamine react with DNA?